[GL3310] GoPAGE™ Bis-Tris Precast Gel (Midi, 12 wells, 12%), 10 gels

Call For Price




 

Description 

GoPAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows GoPAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Tris-Glycine gels. GoPAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-well formats.

GoPAGE™ Bis-Tris Precast Gels are available in Mini (10 x 8.3 cm) and Midi (10 x 10 cm) cassette sizes, which are compatible with most popular electrophoresis systems. GoPAGE™ Mini (GL2XXX) Gels are suitable for Bio-Rad® and other systems. GoPAGE™ Midi (GL3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Hoefer SE260, and other systems.


Key Features

  • User-friendly gel cassette:

    • Easy to use- No comb or tape to remove. 

    • Easy to load samples- Numbered wells; extended and fixed well separator to prevent sample carryover.

    • Easy to monitor- Transparent reference lines on the gel cassette help to monitor electrophoresis.

  • Unique gel formula: 

    • Sharpness- Enhances band sharpness

    • Long shelf life- Up to 12 months when stored at 4°C

  • Broad compatibility: 

    • Wide separation range- Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatibility- Two cassette sizes suitable for most mini-gel tanks.


Storage and stability

Store GoPAGE™ Precast Gels at 4°C for periods up to 12 months. 
Do not freeze GoPAGE™ Precast Gels.
 

 
 

Features of GoPAGE™ Precast Gel

User-friendly gel cassette:

  • Easy to use- No comb or tape to remove. 

  • Easy to load samples- Numbered wells; extended and fixed well separator to prevent sample carryover; loading volume up to 40 μl/wells.

  • Easy to monitor- Transparent reference lines on the gel cassette help to monitor electrophoresis.

 
 

Elasticity of GoPAGE™ Precast Gel

The high elasticity of GoPAGE™ allows researchers easy to handle during post-electrophoresis analysis.

Clear and sharp bands, high resolution

GoPAGE™ Bis-Tris Precast Gel shows high resolution of protein separation.


 


 
 

Setting Up and Running GoPAGE™ Mini Precast Gel

 

 
 

Tips for removing GoPAGE from cassette

 
 

Setting up GoPAGE gel/membrane sandwich for Western transfer

 

 

Recommendations/Tips for Gel Running

1.      Always use fresh 1X running buffer for the inner cathode chamber.

2.      Before sample loading, rinse the wells to remove storage buffer.

3.      Do not use Tris-Glycine running buffer for GoPAGE™ Bis-Tris Precast Gels.

4.      After gel running, keep gel under moisture, and carefully detach the gel from cassette with water.

 

Sample Preparation for SDS-PAGE

1.      Mix protein sample with 2X sample buffer. 

2.      Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.

3.      Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)

 

Prepare GoPAGE™ for Sample Loading

1.    Open the plastic bag of GoPAGE™ Precast Gel.

2.    Briefly rinse the gel cassette with ddH2O and throw out the gel storage buffer within the wells. Avoid squeezing the gel.

3.    Adapt GoPAGE™ to electrophoresis system; instructions are provided below. (XCell SureLock® Mini-Cell Electrophoresis System is recommended.)

4.    Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.

5.    Fill the wells with running buffer prior to sample loading.

6.    Load samples and pre-stained protein marker into numbered wells.

7.    Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.

 

Power Setting for Running GoPAGE™

Optimize the voltage and running time if needed.

 

100 V

150 V

200 V*2

Running Time*1

95-115 mins

65-85 mins

40-60 mins

Expected Current

Initial (per gel)

Final (per gel)

 

15-20 mA

5-10 mA

 

50-60 mA

20-30 mA

 

100-110 mA

30-40 mA

Expected temperature

25-30°C

25-35 °C

25-35°C

*Running time varies depending on gel percentage, running buffer, temperature, and power supply.

*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.

 

Remove GoPAGE™ Gel from Cassette

Open cassette immediately after electrophoresis. Avoid gel drying.

1.    Insert the cassette opener into corners of cassette.

2.    Sequentially pry the opener to separate the two plates.

3.    Gently pull two plates apart from the top of cassette, allowing gel to rest on one plate. If necessary, use gel remover and water flow to help gel rest on one plate.

4.    Carefully detach the gel from the plate with water flow and gel remover.

 Under water flow, insert gel remover between gel and cassette from the well site of gel.

 Slowly push gel remover to the bottom of gel until gel is fully detached.

 Avoid diagonally peeling the gel from the corner.

 If necessary, cut well separators with gel remover.

5.    Gently remove the gel for further staining or Western blotting.



 

 

Troubleshooting Guidelines

Problem

Possible Cause

Suggested Solution

Bubbles between gel and cassette

Gel has been frozen or stored at wrong temperature.

Store GoPAGE Precast Gels at 4°C.

Buffer leaking from the inner chamber

Untight assembly of gels to the electrode modules

Reassemble GoPAGE gels into the electrode modules.

Fill outer chamber with 1X running buffer to the highest level.

Samples do not sink into the wells.

Residual gel storage buffer in the wells

Rinse the gel wells with ddH2O or 1X running buffer before loading.

Insufficient sample buffer

Use more sample buffer to prepare samples.

Gels run faster or more slowly than expected.

Incorrect running buffer

Check buffer composition.

Use fresh 1X running buffer for inner chamber.

Crooked bands at middle or bottom of gel

Gel has been frozen or stored at wrong temperature.

Store GoPAGE Precast Gels at 4°C.

Incorrect running buffer

Check buffer composition.

Use fresh 1X running buffer for inner chamber.

Band pattern curves toward one or both sides of gel.

Buffer leaking from the inner chamber

Check assembly of gels into the electrode modules.

Excessive heating of gel

Check buffer composition. Or dilute running buffer to 0.5-0.75X.

Do not exceed recommended running conditions.

Insufficient buffer in inner or outer buffer chamber

Fill inner and outer chambers to completely cover gel wells.

Poor resolution or fuzzy bands

Excessive heating of gel

Check buffer composition.

Do not exceed recommended running conditions.

Incorrect running buffer

Check buffer composition.

Bands are missing on the membrane after Western transferring.

Proteins move in the wrong direction

Check the order of gel/membrane sandwich assembly, the direction of transfer cassette in transfer modules, and the polarity of connections to power supply.

Swirls or missing bands; bands trail off in multiple directions on the membrane after Western transferring.

Contact between the membrane and the gel was poor; Air bubbles or excess buffer remains between the blotting membrane and the gel. 

Use thicker/more filter paper in the gel/membrane sandwich

Remove air bubbles and excess buffer between gel and membrane by carefully moving the roller over the membrane.

Apparent molecular sizes of prestained protein markers are different as indicated.

Prestained protein markers used have not been calibrated 
for use with GoPAGE gels. Dyes for staining protein markers affect the migration patterns of prestained proteins in different buffer systems.

Calibrate prestained protein markers against unstained proteins of known size or use SMOBIO’s ExcelBand™ Protein Markers.


 GoPAGE™ Precast Gel 

Gel Type

Bis-Tris

TGN (Tris-Glycine-Novel)

Buffer systems

MOPS, MES

Tris-Glycine (Laemmli)

Features

Clear and sharp bands, high resolution

Quick running, clear bands

Gel percentage

8% (GL2110)

12% (GL2310)

4-12% (GL2510)

8% (GL3110)

12% (GL3310)

4-12% (GL3510)

10% (GL4210)

4-15% (GL4510)

10% (GL5210)

4-15% (GL5510)

Cassette size

Mini Gel (10 x 8.3 cm)

Midi Gel (10 X 10 cm)

Mini Gel (10 x 8.3 cm)

Midi Gel (10 X 10 cm)

Electrophoresis system

Bio-Rad systems

Xcell SureLock,

Hoefer SE260

Bio-Rad systems

Xcell SureLock,

Hoefer SE260

Wells, loading volume

12 wells, 40 μl/well

12 wells, 30 μl/well

12 wells, 40 μl/well

12 wells, 30 μl/well

Content

10 gels/Box, cassette opener, product information, Tips card, Gel Remover, [MOPS powder (BT)]

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 3 for three columns

FluoroStain™ Protein Fluorescent Staining Dye

  • Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.

  • High sensitivity — detection level achieve ~3 ng, similar to silver staining

  • Substitution of the Coomassie Blue protein staining method