[DM4160] FluoroBand™ XL 25 kb Fluorescent DNA Ladder, Broad Range (up to 25 kb), 500 μl

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Description 

The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. 


Features

  • Sharp bands

  • Quick reference enhanced bands 

  • Ready-to-use— premixed with loading dye for direct loading

  • Stable— room temperature storage over 6 months

  • Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye


Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.


Range 

250 ~ 25,000 bp 


Concentration 

51.6 µg/ 500 µl 


Recommended loading volume 

5 µl/ well


Storage

Protected from light 
Room temperature for 6 months
4°C for 12 months 
-20°C for 24 months 

 

Specification

Cat. No.

DM4160

Series Name

FluoroBand™

Product Size

500 μl

Size Range

250 – 25000 bp

Band Number

14

Tracking Dye

Bromophenol blue 
and Xylene cyanol FF

Enhanced Band

1000 and 3000 bp

 


 


Odoo - Sample 3 for three columns

FluoroDye™ DNA Fluorescent Loading Dye

  • Excellent for premix with DNA sample 

  • Sensitivity up to 0.14 ng DNA

  • Safety dye

  • Convenience - monitor the electrophoresis in real-time

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng DNA or 1 ng total RNA

  • A safer alternative to EtBr 

  • Suitable to blue or UV light 


Odoo - Sample 3 for three columns

B-BOX™ Blue Light LED Epi-illuminator

  • 470 nm long wavelength

  • Improved cloning efficiency 

  • Compact, light-weight, and portable (less than 1 kg)

  • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation