ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX)
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step qPCR Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step qPCR Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.
ExcelRT™ Reverse Transcriptase
The ExcelRT™ Reverse transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, a RNA dependent DNA polymerase capable of generating the first strand cDNA using RNA template.
ExcelRT™ 2X One-Step RT-PCR Kit
The ExcelRT™2X One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target gene from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides users an alternative to two lengthy steps (first strand generation and amplification) by using a single mixture in a single tube for the one-step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+, enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ 2X One-Step RT-PCR Kit allows users to complete the RT-PCR process using a thermocycler in a single reaction setting and is ideal for target RNA amplification. This product is capable of detecting even trace amounts of target RNA.
ExcelRT™ Reverse Transcription Kit
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
ExcelRT™ Reverse Transcription Kit II
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative of the lengthy two steps – first strand generation, amplification solution by using a single mixture, single tube, one step reaction.
- RI1000 / RI1001, RNAok™ RNase Inhibitor
RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by
affinity chromatography from E. coli
. This protein inhibits pancreatic-type ribonucleases, RNase
A, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™
RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic
RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV,
M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control
tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other
commercially available preparations of RNase inhibitors.